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Established A Index Adapters Make reference to Index 2 (i5) Orientation for more information regarding how to enter i5 bases about the sample sheet in ahead or reverse enhance orientation.
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For Neighborhood Run Supervisor (LRM), input the sequences within the reverse enhance when starting the run so the software quickly results in the forward orientation as needed for Assessment. This is the alternative of the vast majority of our assistance and is just for the MiniSeq Speedy kits.
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Index 2: the i5 index column is within the reverse enhance orientation (are classified as the reverse complement with the i5 indexes during the sample sheet).
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The sequences displayed are what's entered into the ultimate sample sheet. Kit definitions provide the i5 while in the forward orientation. The i5 sequences more info are going to be properly reverse complemented and displayed in the IEM interface based upon instrument/reagent variety.